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Image Search Results
Journal: Oncotarget
Article Title: Fatty acid binding protein 4 enhances prostate cancer progression by upregulating matrix metalloproteinases and stromal cell cytokine production
doi: 10.18632/oncotarget.22908
Figure Lengend Snippet: (A) Histogram showing the relative value (%) of invading cells of each condition based on untreated PC-3 cells. PC-3 cells were treated with 50 nM FABP4 siRNAs or rFABP4 at the indicated concentration. All invading cells were counted and compared with untreated PC-3 cells (Ctrl); * P < 0.05 and ** P < 0.01. (B, C, D and E) Altered matrix metalloproteinase (MMPs) levels by FABP4. (B and C) Overall, 1 × 10 5 PC-3 cells were cultured in a 35-mm dish, and treated with 50 nM FABP4 siRNA-1. Each conditioned medium (CM) was then collected and analyzed for MMP levels using the RayBio Human MMP Antibody Array kit. The signal intensity in the array images was quantified and scored by Densitograph software (ATTO). Fold changes among samples were analyzed and compared using RayBio Antibody Array Analysis Tool provided by RayBiotech (B). MMP2 and MMP9 signals in the array images are indicated by rectangular boxes (C). Fold changes of signals in control PC-3 cells (upper) and FABP4 siRNA-1 treated PC-3 cells (lower) were analyzed using the RayBio Antibody Array Analysis Tool (C). (D) Zymography analysis of MMP2 and MMP9 activity. MMP2 and MMP9 enzymatic activity in condition medium from PC-3 cells treated with 50 nM FABP4 siRNAs or 100 ng ml -1 rFABP4. (E) Relative MMP2 and MMP9 mRNA levels. PC-3 cells were treated with 50 nM FABP4 siRNAs and 100 ng ml -1 rFABP4 for 24 hours. MMP2, MMP9 and beta-actin mRNA levels were assessed by quantitative RT–PCR, and compared with untreated cells. Mean ± S.D., * P < 0.05 and ** P < 0.01.
Article Snippet: The fold changes among samples were calculated analyzed using the
Techniques: Concentration Assay, Cell Culture, Ab Array, Software, Zymography, Activity Assay, Quantitative RT-PCR